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Faculty

SAITO Shingo
Professor

Contact Information
Email
Websites

https://www.apc.saitama-u.ac.jp/bunseki/en/staff.html


Summary of Activities

Selection of Functional DNA Aptamers by Capillary Electrophoresis; Capillary Electrophoresis for Actinide Ions; Gel Electrophoresis for Metalloproteins and Humic Substances; Capillary Electrophoresis for Microbes; Fluorescent Probe for Saccharide Recognition.

Education

B. Eng, Tohoku University, 1996
M. Eng, Tohoku University, 1998
Ph.D., Tohoku University, 2001

Professional Experiences

Assistant Professor, Kitami Institute of Technology (Hokkaido, Japan), 2001-2007
Visiting researcher, RIKEN (Saitama, Japan), 2005-2008
Associate Professor, Graduate School of Science and Engineering, Saitama University (Saitama, Japan), 2007-2017
Professor (P.I.), Graduate School of Science and Engineering, Saitama University (Saitama, Japan), 2017-present
Visiting researcher, Wake Forest University, (NC, USA), 2010-2011

Lectures

Analytical Chemistry I
Exercise of Analytical Chemistry
Analytical Chemistry II
Experiments in Applied Chemistry IV
Advanced Analytical Chemistry I (MC)
Analytical Separation Chemistry II (DC)

Honors and Awards

Shigeru Terabe Award, "Electrophoresis to find chemical species with specific interactions" (2023).
Hot Article Award, Analytical Sciences, "Holo/apo conversion two-dimensional urea PAGE for separation of Fe3+-bound transferrin in serum " (2024).
Masao Horiba Award, "Electrophoresis for determination of ultratrace heavy metal ions in radioactive wastes and environmental microbes using novel fluorescent probes" (2013).
Advanced Engineering Science Award, Japan Atomic Energy Agency (2013)
Best Poster Award in HPLC 2011, "Highly Emissive Metal Probes Suitable for Ultratrace Detection of Lanthanide and Actinide Ions by Capillary Electrophoresis-Laser-Induced Fluorescence" (2011)
Japan Society for Analytical Chemistry Award for Younger Researchers, "Total Design of Novel Fluorescent Probes and Kinetically Integrated Chemical System for Separation and Detection of Metal Ions" (2008)
Encouraging Prize of Analytical Chemistry in JSAC Hokkaido, 2004, "High Performance Separation System Using Kinetically Inert Metal Chelates and Their kinetics" (2005)

Selected Publications

S. Saito* et al., “Unusually Kinetically Inert Monocationic Neptunyl Complex with a Fluorescein-Modified 1,10-Phenanthroline-2,9-dicarboxylate Ligand: Specific Separation and Detection in Gel Electrophoresis,” Inorganic Chemistry, 62, 730-738 (2023). Selected as a supplemental cover.


S. Saito* et al., "Single-Round DNA Aptamer Selection by Combined Use of Capillary Electrophoresis and Next Generation Sequencing: An Aptaomics Approach for Identifying Unique Functional Protein-Binding DNA Aptamers," Chemistry - A European Journal, 27, 10058-10067 (2021). Selected as an inside cover.


S. Saito* et al., "Stoichiometry between Humate Unit Molecules and Metal ions in Supramolecular Assembly Induced by Cu2+ and Tb3+ Measured by Gel Electrophoresis Techniques," Environmental Science & Technology, 55, 15172-15180 (2021).


S. Saito* et al., "Advanced Gel Electrophoresis Techniques Reveal Heterogeneity of Humic Acids Based on Molecular Weight Distributions of Kinetically Inert Cu2+-Humate Complexes," Environmental Science & Technology, 53, 14507-14515 (2019).


S. Saito* et al., "Two-dimensional Polyacrylamide Gel Electrophoresis for Metalloprotein Analysis Based on Differential Chemical Structure Recognition by CBB Dye," Scientific Reports, 9, 10566 (2019).


S. Saito* et al., "Rapid Acquisition of High-Affinity DNA Aptamer Motifs Recognizing Microbial Cell Surfaces Using Polymer-Enhanced Capillary Transient Isotachophoresis," Chemical Communications, 52, 461-464 (2016). Selected as an inside back cover.


S. Saito* et al., "Molecular Design of Boronic Acid-Functionalized Squarylium Cyanine Dyes for Multiple Discriminant Analysis of Sialic Acid in Biological Samples: Selectivity Toward Monosaccharides Controlled by Different Alkyl Side Chain Lengths," Analytical Chemistry, 87, 1933-1940 (2015).


S. Saito,* "A New Molecular Motif for Recognizing Sialic Acid Using Emissive Lanthanide-Macrocyclic Polyazacarboxylate Complexes: Acid-Dissociation of A Coordinated Water Molecule Controls Selectivity," Inorganic Chemistry, 52, 6239-6241 (2013).


S. Saito et al., "On-Column Labeling of Gram-Positive bacteria with a Novel Boronic Acid Functionalized Squarylium Cyanine Dye for Analysis by Polymer-Enhanced Capillary Transient Isotachophoresis," Analytical Chemistry, 84, 2454-2458 (2012).

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